Protocol — Quantitative Real-Time PCR (qRT-PCR): 3K-SNCA cells v1
Gene expression analysis in differentiated and undifferentiated 3K-SNCA SH-SY5Y cells using Qiagen RNA extraction and reverse transcription, followed by TaqMan® qRT-PCR on the QuantStudio® 1 platform.
Authors
- David Chau (ORCID: https://orcid.org/0000-0001-9200-6749)
- Anthony H.V. Schapira (ORCID: https://orcid.org/0000-0002-3018-3966)
- Giuseppe Uras (ORCID: https://orcid.org/0000-0003-3494-8521)
- Saionara Siqueira Lucas
- Sofia Koletsi (ORCID: https://orcid.org/0000-0002-6661-7797)
- Federico Fierli (ORCID: https://orcid.org/0000-0002-8259-2067)
Institutions
- Anthony Nolan (GB)
- University College London (GB)
Publication Details
- Published
- 2025-10-13
- DOI
- https://doi.org/10.17504/protocols.io.x54v95zbzl3e/v1
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00