Study on a growth promoting effect and mechanism of the extract of stem and leaf from <i>Atractylodes lancea</i> DC. on nile tilapia
Atractylodes stems and leaves, often discarded as waste, were investigated for their growth-promoting effects and chemical composition. This study evaluated <i>Atractylodes lancea</i> DC. extract (ALE) on <i>Lactiplantibacillus plantarum</i> growth, with optimal treatment at 7.5 mg/mL for 24 h. ALE's effects on Nile tilapia were also assessed using 120 juvenile fish fed diets with different ALE doses (0.2%, 0.4%, 0.8%) for 31 days. Results showed that ALE significantly improved fish growth, survival, and intestinal health. Network pharmacology suggested that key compounds (rutin, luteolin) promoted growth through the PI3K-Akt pathways. Rutin and luteolin in ALE were confirmed by HPLC with authentic standards. RT-qPCR further showed that ALE activated the Grb2/PI3K/Akt signalling pathway in tilapia, supporting the predicted growth-related mechanism, and highlighting ALE's potential for sustainable aquaculture.
Authors
- Ziying Wang (ORCID: https://orcid.org/0000-0003-2915-1120)
- Wei Tian (ORCID: https://orcid.org/0009-0006-9568-7600)
- Zhiwei Huang (ORCID: https://orcid.org/0000-0002-8201-9391)
- Dongdong Shi (ORCID: https://orcid.org/0000-0003-3585-7664)
- Xiumei Li (ORCID: https://orcid.org/0000-0001-8862-8860)
- Yuefei Liu (ORCID: https://orcid.org/0009-0008-0671-8298)
Institutions
- Inner Mongolia Agricultural University (CN)
- Tianjin University of Science and Technology (CN)
- Inner Mongolia Electric Power (China) (CN)
- Shandong Institute for Product Quality Inspection (CN)
- Feed Research Institute (CN)
Publication Details
- Journal
- Natural Product Research
- Published
- 2025-10-13
- DOI
- https://doi.org/10.1080/14786419.2025.2570511
- Primary Topic
- Ginseng Biological Effects and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00