Protective Role of Superoxide Dismutase Assay and Its Relation with Wrightia Tinctoria R.Br Leaf Extract and Its Correlation with Bioactive Compounds

Wrightia tinctoria R.Br is a medicinal plant valued for the bioactive compounds and the diverse pharmacological properties referring to antiinflammatory, skin diseases, acne, anti-bacterial, anti-diabetic, anti-HIV properties. This study aimed to do the pre-extraction process followed by the extraction and perform the preliminary analysis along with the anti-oxidant potential of the defatted ethanolic leaf extract of Wrightia tinctoria R.Br using Soxhlet apparatus and the extract was evaporated in rotary evaporator to a syrupy consistency and was concentrated to extract. The phytochemical screening revealed important Phyto constituents like terpenoids, phenols, flavonoids carbohydrates that have role in diverse pharmacological spectrum. The antioxidant assay using Superoxide dismutase unravels the key foundation for many other investigations in relation to complex diseases that rises from oxidative stress .These findings indicate that the leaf extract possesses significant scavenging of superoxide radicals (O2) by converting to less damaging hydrogen peroxide and molecular oxygen, likely due to its phenolic and flavonoid content along with the terpenoid compounds while the study provides insight into several phytoconstituents and anti-oxidant efficacy. Further research into in-silico and in-vivo studies are necessary and the proteomic studies in relation to these actions may untie the different cellullar and molecular mechanisms.