A Thermally Active Tannase from Tea Endophyte <i>Paraburkholderia pallida</i> Exhibits High Specificity for Galloylated Catechins
Tannases hydrolyze ester bonds in hydrolyzable tannins and galloylated compounds with significant applications in food and agricultural industries. However, the diversity of tannase activities among tea plant-associated bacteria remains underexplored. Here, we identified Tan<sub>pp</sub>, a novel tannase/feruloyl esterase from the endophyte <i>Paraburkholderia pallida</i> XY820. Tan<sub>pp</sub> displayed optimal activity against (-)-epigallocatechin 3-gallate (EGCG) at 60 °C and pH 6.0, with thermal activation upon brief preincubation at 40-45 °C. The enzyme demonstrated high specificity for galloylated catechins. Structure-guided mutagenesis yielded Y338A and R304A variants with 3.09- and 1.89-fold increased catalytic efficiency toward EGCG, respectively. This work advances our understanding of tannase function within tea plant microbial communities and positions Tan<sub>pp</sub> and its engineered derivatives as promising biocatalysts for tea polyphenol biotransformation and functional food applications.
Authors
- Xiaomin Yu (ORCID: https://orcid.org/0000-0002-8650-6795)
- Shuang Guo (ORCID: https://orcid.org/0000-0002-7344-6121)
- Tingting Lin (ORCID: https://orcid.org/0000-0003-4005-9181)
- Zhenghua Du
- Ruimei Wu
- Xiaoxia Wang (ORCID: https://orcid.org/0000-0001-5094-0269)
- Xinyu You (ORCID: https://orcid.org/0009-0008-6739-5605)
- Qiyu Li (ORCID: https://orcid.org/0009-0002-7308-1334)
- Fuquan Gao
- Kai-Qiang He
- Li Liu
Institutions
- Henan Institute of Science and Technology (CN)
- Fujian Agriculture and Forestry University (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2025-10-13
- DOI
- https://doi.org/10.1021/acs.jafc.5c08942
- Citations
- 1
- Primary Topic
- Tannin, Tannase and Anticancer Activities
- Type
- article
- Field-Weighted Citation Impact
- 1.26