Resistance Analysis of Vibrio сholerae Isolated in Russia to Quinolones and Beta-Lactams

The circulation of antibiotic-resistant strains of Vibrio cholerae in the world requires dynamic monitoring. The aim of the study was to assess the level of sensitivity/resistance of V. cholerae to representatives of quinolones (including fluoroquinolones) and beta-lactams. Material and methods. The serial dilution method was used to determine the minimum inhibitory concentrations (MICs) of nalidic acid, ciprofloxacin, ampicillin, and ceftriaxone for 682 strains of V. cholerae isolated in Russia in 2005–2020 from humans and environmental objects. Results. All toxigenic strains and 20.4 % of non-toxigenic strains were resistant to nalidixic acid; 30 to 50 % of toxigenic strains and 5.4 % of non-toxigenic strains were resistant to ceftriaxone. No resistance to imipenem was detected. In the presence of resistance genes to fluoroquinolones and beta-lactams, about half of the cultures were characterized by sensitivity to these antibiotics in vitro. Consequently, there is a risk of the emergence of antibiotic-resistant forms of the cholera pathogen during the process of etiotropic therapy of the infection, which dictates the need to monitor the sensitivity/resistance of vibrios to fluoroquinolones or beta-lactams during treatment with these antibiotics.