In vitro cultivation and preservation of Entamoeba histolytica on TYI-S-33 medium

An enormous protozoan parasite found worldwide, particularly in unsanitary areas, is called Entamoeba histolytica, and considered the main causes for intestinal infection Amoebiasis is worldwide in distribution and is the third most common cause of death due to parasitic infection after malaria and schistosomiasis. In the current study, stool samples were taken from a total of 270 patients who were attending to Azadi Teaching Hospital of Kirkuk City. The participants aged from (3 month to 79 years old) that suffering diarrhea, abdominal pain, vomiting, dehydration and, flatulence. The study was done between October 2022 and April 2023. By general stool examination identified macroscopically colors that are classified to yellowish, brownish, greenish and bloody. and consistency for liquid, soft and solid as well as microscopic examination for presence of blood and pus cell and identified cyst or trophozoite or both of E. histolytica and then isolate and cultivated and maintaining in TYS-S-33 media selective for E. histolytica and add healthy human inactivated serum instate of bovine serum to the media.