Illuminating radionuclide therapy response: Monitoring cancer cell and CAF survival in a direct 2D co-culture model

We successfully established 2D co-culture models of PDAC and BC that include CAFs neighboring the cancer cells, as is the case in the patient tumor microenvironment. The use of dual-color reporter genes enabled efficient, cell-type-specific analysis of radionuclide therapy efficacy via BLI. The developed models provide a user-friendly platform for evaluating therapeutic responses and can be further refined into 3D systems for even higher patient resemblance.