The Effect of The Use of Multicomponent Binder Toxin on Performance, Liver Condition and Quantitative and Qualitative Traits of Eggs at The End of The Production Period of Laying Hens

Background: As the age of laying hens increases, the percentage of diseases such as fatty liver and osteoporosis increases, which is accompanied by a decrease in reproductive performance.Fatty liver disease is one of the major problems in rearing laying hens at the end of the period, which is associated with a sharp decrease in production.For this purpose, toxin binder is used in order to increase the digestibility and prevent the adverse effects of toxins on the liver.The use of multi-component toxin binder can have more effects on the elimination of toxins.Many studies have evaluated the positive use of herbal compounds such as milk thistle, chicory powder, turmeric, and artichoke as toxin binders in the diet.This experiment was designed to investigate the effects of Bentomax Herbal Chitica toxin binder, which contains a combination of two medicinal plants (thyme and Silybum marianum), yeast cell wall (beta-glucan and mannan oligosaccharide), and aluminosilicate compounds, on performance traits, fatty liver syndrome, and egg quantitative and qualitative traits in late-stage production of high-line w80 laying hens.Methods: In order to investigate the effect of different levels of multicomponent binder toxin on yield, quantitative and qualitative egg traits and fatty liver status in laying hens at the end of the production period, an experiment was designed, in which 630 laying hens of high-line W80 strain in 5 treatments and 6 repetitions and 21 pieces in each repetition were divided in a completely random design.The experimental treatments included 1-control, and from 2 to 5, respectively: 0.5, 1, 1.5 and 2 g/kg binder toxin.The test period included two weeks of equalization and record taking to equalize the treatments and two 28-day periods from 87-91 weeks and 92-95 weeks .On the last day of the experiment, one bird was randomly selected from each replicate, and after weighing, it was euthanized.The internal organs (liver, abdominal fat, spleen, gallbladder, and pancreas) were separated and weighed separately using a digital scale with an accuracy of 0.01 g and their percentages relative to body weight were calculated.Fatty liver was scored from 0 to 5. Liver hemorrhage was also scored based on 0 to 3, with a score of 0 indicating no bleeding, a score of 1 indicating a maximum of 10 blood spots, a score of 2 indicating more than 10 blood spots, and a score of 3 indicating obvious bleeding.To measure the activity of liver enzymes, a piece was selected from each replicate at the end of the period and a blood sample was obtained from the wing vein and immediately centrifuged at 3000 rpm for 10 minutes serum was separated and the samples were stored at a temperature of minus 20 degrees.An autoanalyzer was used to measure the activity of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase enzymes.Results: The results of this experiment showed that the addition of levels of 0.5, 1, 1.5 and 2 g/kg of multicomponent binder toxin caused a significant decrease in feed consumption and feed conversion ratio compared to the control group.The percentage of egg production at 92-95 weeks in the group receiving 1.5 g/kg toxin binder was significantly higher than the control group.The eggshell percentage was significantly higher in the groups receiving Binder toxin than in the control group.The egg yolk percentage was also lower in the group receiving 2 g/kg of binder toxin than the control group.The percentage of liver and abdominal fat decreased under the influence of the experimental treatments compared to the control group.The liver color index in the groups receiving binder toxin was significantly higher than the control group, and the bleeding index was lower than the control group.Liver enzymes (alanine aminotransferase, aspartate