A protocol for real-time tracking of transposon products and host proteins in Drosophila ovaries

Drosophila ovary is an excellent model for studying material transportation and dynamics. Here, we present a protocol for live imaging of transposon products and host proteins in Drosophila ovaries. We describe steps from ovary dissection, ovariole isolation, and mounting to live tracking of fluorescently labeled proteins with single-cell resolution. Furthermore, we detail procedures of image processing for jitter correction and kymograph analysis. This protocol can be applied to studies on the dynamics of other fluorescently tagged proteins in Drosophila ovaries. For complete details on the use and execution of this protocol, please refer to Shen et al.¹.