Protocol to identify and sort antigen-specific B cells from Plasmodium-infected mice
Here, we detail a protocol to identify an antigen-specific B cell population using SpyCage reagents tailored to bind MSP<sub>1-19</sub> antigen in a rodent malaria model. We describe steps for the preparation of splenic single-cell suspension, B cell enrichment, and staining to facilitate sorting of live cells for downstream applications such as single-cell RNA, V(D)J, and assay for transposase-accessible chromatin (ATAC) sequencing. For complete details on the use and execution of this protocol, please refer to Calôba et al.<sup>1</sup>.
Authors
- Scott E. Lindner
- Rahul Vijay (ORCID: https://orcid.org/0000-0001-5303-929X)
- Allen M. Minns
- Carolina Calôba (ORCID: https://orcid.org/0000-0003-4723-2127)
Institutions
- Pennsylvania State University (US)
- Rosalind Franklin University of Medicine and Science (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-10-07
- DOI
- https://doi.org/10.1016/j.xpro.2025.104129
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 0.00