MALDI-MSI Lipidomics of Lung Tissue v1

This protocol describes the procedure to obtain MALDI mass spectrometry images of lipids using a high mass resolution FTICR. This protocol is optimized for 12 µm sections of agarose-inflated and carboxymethyl cellulose-embedded fresh-frozen lung tissues. The embedding method is described in Lukowski et al. doi.org/10.3389/fmolb.2022.1022775

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Published
2025-03-21
DOI
https://doi.org/10.17504/protocols.io.n2bvjdojpvk5/v1
Primary Topic
Metabolomics and Mass Spectrometry Studies
Type
article
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article

MALDI-MSI Lipidomics of Lung Tissue v1

Christopher Anderton, Brittney L. Gorman
Metabolomics and Mass Spectrometry Studies
article

MALDI-MSI Lipidomics of Lung Tissue v1

Christopher Anderton, Brittney L. Gorman
article en

Abstract

This protocol describes the procedure to obtain MALDI mass spectrometry images of lipids using a high mass resolution FTICR. This protocol is optimized for 12 µm sections of agarose-inflated and carboxymethyl cellulose-embedded fresh-frozen lung tissues. The embedding method is described in Lukowski et al. doi.org/10.3389/fmolb.2022.1022775

Pacific Northwest National Laboratory (US)
Openalex Percentile: Top 75%
Metabolomics and Mass Spectrometry Studies
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MALDI-MSI Lipidomics of Lung Tissue v1 — Christopher Anderton, Brittney L. Gorman · (2025) | TGRS Research Map | TGRS