Structural and Functional Characterization of Anti-SARS-CoV-2 Spike Monoclonal Antibodies Produced via Bicistronic Expression in CHO Cells
Together, these results provide insight into the impact of gene positioning within bicistronic vectors on mAb expression efficiency and quality, supporting optimization strategies for scalable recombinant antibody production.
Authors
- Federico F. Marsili (ORCID: https://orcid.org/0000-0002-5376-2071)
- Guilherme A. P. de Oliveira (ORCID: https://orcid.org/0000-0002-0063-5888)
- Kátia Maria dos Santos Cabral (ORCID: https://orcid.org/0000-0003-0817-4884)
- Renato Sampaio Carvalho (ORCID: https://orcid.org/0000-0003-2530-752X)
- Leda R. Castilho (ORCID: https://orcid.org/0000-0002-5904-410X)
- Andréa Queiróz Maranhão (ORCID: https://orcid.org/0000-0002-1946-7191)
- Hiam R. S. Arruda (ORCID: https://orcid.org/0000-0002-0948-5484)
- Mayra A. Marques (ORCID: https://orcid.org/0000-0002-4131-4634)
- Marcius S. Almeida (ORCID: https://orcid.org/0000-0003-4921-8185)
- Fernanda Bittencourt de Aquino (ORCID: https://orcid.org/0009-0008-8233-3330)
Institutions
- Universidade Federal do Rio de Janeiro (BR)
- Universidade de Brasília (BR)
- Instituto Federal do Rio de Janeiro (BR)
- Institute of Physics (AZ)
- National Institute of Science and Technology for Structural Biology and Bioimaging (BR)
Publication Details
- Journal
- Antibodies
- Published
- 2025-10-09
- DOI
- https://doi.org/10.3390/antib14040086
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
- Universidade Federal do Rio de Janeiro