Applicability of the constitutive GAP promoter for recombinant human insulin hormone production in Pichia pastoris

Insulin is a hormone regulates glucose homeostasis in the body.In this study, the precursor of the human insulin hormone was expressed under the constitutive Glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter.The expression vector was transferred into the P. pastoris X33 strain by electroporation.After the protein expression study in 5L scale bioreactor, the samples were analysed by SDS-PAGE and ELISA methods.In this study, 11 mg/ L of protein was produced in the bioreactor at the end of production.The MALDI-TOF analysis confirmed that the protein produced was a precursor of the human insulin hormone.At the end of the study, it can be said that a bioprocess has been developed under bioreactor conditions to produce human insulin precursors by using constitutive GAP promoter of P. pastoris.