Diagnostic Utility of PRAME in Differentiating Melanocytic Lesions, Especially Spitzoid Tumors

Objectives: Differential diagnosis of melanoma and nevi is usually straightforward. However, there can be difficult cases, and additional reliable methods are needed for differentiating benign melanocytic lesions from malignant ones. We aimed to investigate the role of PRAME in challenging melanocytic lesions. Materials and Methods: The study included 27 atypical Spitz tumors, 12 Spitzoid melanomas, 20 conventional melanomas, and 10 high-grade dysplastic nevi. The control group included 20 melanocytic nevi, 20 Spitz nevus, and 10 low-grade dysplastic nevus. Immunohistochemistry for PRAME, BRAF, p16, BAP1, MelanA, and Ki67 was performed using automated systems. Staining of ≥75% was defined as PRAME positive. Data were analyzed using SPSS v11.5, and a p-value