Hepato-curative Potential of Methanolic Leaf Extract of Anisopus mannii in CCl4-Induced Hepatotoxic Rats

Aim: To investigate the hepato-curative effects of the methanolic leaf extract of Anisopus mannii. Study Design: An in vivo experimental study using CCl4-induced hepatotoxic albino rats to evaluate the hepato-curative effects of the methanolic leaf extract of Anisopus mannii. Place and Duration of Study: Department of Biochemistry, Umaru Musa Yar’adua University, Katsina, between December 2023 and May 2024. Methodology: The leaf extract was evaluated for the presence of phytochemicals. The extract was subjected to thin layer chromatography, and the fractions obtained were fed to the hepatotoxic wistar albino rats. Results: Phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids, phenols and terpenoids. Thin layer chromatography revealed four (4) fractions. The hepato-curative effect was evaluated in the hepatotoxic albino rats over three weeks. The fractions significantly improved alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and others compared to hepatotoxic control group. These effects were comparable to the standard drug Silymarin. Conclusion: This study showed that the methanolic leaf extract of Anisopus mannii possesses hepato-curative potential which could be beneficial in the treatment of hepatotoxicity. Fraction 3 and Fraction 4 were found to be the most effective in terms of the hepatp-curative potential.