Phytochemical Profiling of Wrightia Tinctoria Leaf and Identification of Marker Compound Rutin by RP-HPLC Analysis for the Treatment of Psoriasis

Background Psoriasis is a skin disorder characterized by hyperproliferation of skin cells and an inflammatory response involving neutrophils. Wrightia tinctoria has traditionally been used in treating psoriasis and various studies have supported its effectiveness. The study investigated the phytochemical contents of Wrightia tinctoria leaves and determined the existence of rutin a bioactive substance thought to have therapeutic effects on psoriasis.Aim The study aimed to assess the therapeutic potential of Wrightia tinctoria plant leaf extract by assessing its phytochemical composition and determining the marker molecule rutin using Reversed-Phase High-Performance Liquid Chromatography RP-HPLC analysis.Methods Fresh Wrightia tinctoria leaves were collected and analyzed using qualitative and quantitative microscopy. The phytochemical profile was determined using an aqueous extract of dried leaf powder. A sensitive RP-HPLC method was devised to detect the rutin concentration in aqueous leaf extract.Results The phytochemical analysis revealed the presence of alkaloids carbohydrates flavonoids sterols and tannins. A good linearity was observed in the chromatographic data. The retention time of rutin was found to be 3.122 min at 256 nm. The concentration of rutin in the extract was 8.62 microgmL. The reported value of chromatographic parameter was identified by comparing with standard solution having retention time of 3.198 min at 10 microgmL.Conclusion The pharmacognostical evaluation of Wrightia tinctoria leaves provides useful information for standardization protocols. The short retention time in the RP-HPLC method indicates fast and efficient chromatographic separation enabling quicker and more reliable analysis of rutin.nbsp