Studies on Hydrostatic Pressure Responses to Piezo1 and TRPV4 in the Mouse Lens

The lens responds with a negative change of intracellular hydrostatic pressure in cells near the lens surface when Piezo1 is activated. Piezo1 and TRPV4 appear to be independent because they cause additive pressure reduction responses. However, unlike TRPV4, Piezo1 likely plays no role in feedback control of surface pressure because it does not respond to positive pressure changes. When TRPV1-dependent pressure recovery was blocked, activation of either Piezo1 or TRPV4 caused a pressure change that appears consistent with phosphatase-dependent activation of latent Na/K pumps in DF cells.