Establishment and application of nano-liposomes system for targeted therapy of methicillin-resistant Staphylococcus aureus enteritis

Inspired by the damage caused by bacterial pore-forming toxins to cell membranes, this study utilized the film-ultrasound dispersion method and polyethylene glycol (PEG) modification to prepare resveratrol and nisin-loaded nanoliposomes (RN-NPs) using soy lecithin as the main encapsulating material. These RN-NPs were designed for targeted therapy against Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) enteritis. The synergistic inhibitory effects of resveratrol and nisin (RN) on MRSA were confirmed through SEM, FCM, and gel electrophoresis. In addition to being highly stable and resistant to erosion by stomach acid, RN-NPs can also be specifically released to treat MRSA enteritis. In animal experiments, RN-NPs exhibited superior therapeutic effects against MRSA enteritis compared to free RN and positive drugs. They showed a considerable improvement in pharmacodynamic indicators such as intestinal cytokine expression, MPO activity, and histological evaluation scores. Consequently, RN-NPs have been proven to be a very promising targeted drug delivery system for the management of <i>MRSA</i> enteritis. This study has important guiding significance for the future development of targeted drug delivery systems for enteritis.