One‐pot assay for rapid detection of heterozygous herbicide resistance in <scp> <i>Digitaria ciliaris</i> </scp> var. <scp> <i>chrysoblephara</i> </scp> by combining <scp>CRISPR</scp> /Cas and <scp>LAMP</scp>
This method enables real-time one-pot field detection by integrated with miniaturized detection devices, significantly enhancing its practicality and potential for widespread application. This work provides a novel technical approach for detecting herbicide resistance for global weed resistance monitoring and management. © 2025 Society of Chemical Industry.
Authors
- Tao Gu
- Hongchun Wang (ORCID: https://orcid.org/0000-0002-3565-8414)
- Jing Xue (ORCID: https://orcid.org/0000-0002-7331-1382)
- Zichang Zhang (ORCID: https://orcid.org/0000-0001-6523-7613)
- Jingjing Cao (ORCID: https://orcid.org/0000-0001-7335-3876)
- Yang Sun (ORCID: https://orcid.org/0000-0002-8141-6758)
- Zaobing Zhu (ORCID: https://orcid.org/0000-0001-7290-2132)
Institutions
- Jiangsu Academy of Agricultural Sciences (CN)
- Health First (US)
- Institute of Plant Protection (CN)
- Anhui Normal University (CN)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-10-09
- DOI
- https://doi.org/10.1002/ps.70279
- Citations
- 2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.61