Bazı Doğal Mantarların Etanol Ekstraktlarının Anti-MRSA ve Antimikrobiyal Aktivitelerinin Değerlendirilmesi

The aim of this study was to determine the antimicrobial activity of ethanol extracts of Hydnum repandum, Tricholoma fracticum and Russula emetica mushrooms against some standard pathogenic microorganisms and anti-MRSA activity against clinically originated Methycilline Resistant Staphylococcus aureus (MRSA) strains. Broth microdilution method was used to determine the antimicrobial activity. Ethanol extract of H. repandum had antimicrobial activity against standard microorganisms at doses of 0.02 – 3.12 mg/ml, the highest activity was found against Gram positive Bacillus cereus strain with a Minimum Inhibitory Concentration (MIC) value of 0.02 mg/ml. It was also found to have antifungal activity against Candida albicans with a MIC value of 1.56 mg/ml. It was determined that the ethanol extract of H. repandum showed anti-MRSA activity against all clinically sourced MRSA strains at doses ranging from 0.39 to 3.12 mg/ml. The highest antibacterial activity of the ethanol extract of Tricholoma fracticum was similarly determined against B. cereus at a dose of 0.39 mg/ml, while it was observed to have low activity against some of the other strains studied. It was also found that this species did not have antifungal activity. It was seen that it had weak anti-MRSA activity only against four MRSA strains. It was determined that the ethanol extract of Russula emetica had only a weak effect against Salmonella enteritidis at a dose of 3.12 mg/ml, and a moderate effect against B. cereus at a dose of 0.39 mg/ml. It was found that it did not have any antimicrobial activity against other standard strains and C. albicans. It was also seen that it did not have anti-MRSA activity against clinically sourced MRSA strains. It was concluded that the ethanol extract of H. repandum had higher antimicrobial activity against standard pathogenic microorganisms and clinically sourced MRSA strains compared to other species.