A Supramolecular Material for Selective Fluorescence-Based Detection of the Neuromuscular Blocker Rocuronium Bromide

Monitoring Rocuronium Bromide (RcBr), a neuromuscular blocker widely used in anesthesia, is clinically important yet analytically underexplored. We report a fluorescence-based sensor for RcBr using Thioflavin-T (ThT) aggregates templated by γ-sulfated cyclodextrin (γ-SCD). This competitive displacement assay exploits the high binding affinity of Rocuronium for γ-SCD to displace Thioflavin-T from supramolecular aggregates, resulting in a fluorescence turn-off response. The sensor exhibits submicromolar sensitivity (LOD ∼ 0.2 ± 0.02 μM), rapid response (<1 min), and high selectivity over biological interferents. Its functionality in diluted human serum demonstrates potential for possible clinical applications. This work presents a novel optical approach for RcBr sensing that may support future developments in neuromuscular blockade monitoring.