The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease

During initial Lyme disease infection, existing diagnostic tests have poor sensitivity, resulting in a high number of false-negative tests. This is due to the lag between infection and a robust immune response capable of being detected by such tests. With a multiplexed array of nine unique antibody targets specific for <i>Borrelia burgdorferi</i>, interpreted by a proprietary machine learning algorithm, the InBios Lyme Detect Multiplex ELISA has the potential to increase diagnostic sensitivity within the first few weeks of infection, reducing the number of false-negative tests. Improving diagnostic sensitivity during early infection would reduce the risk of developing severe symptoms, including post-treatment Lyme disease.

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Publication Details

Journal
Journal of Clinical Microbiology
Published
2025-10-09
DOI
https://doi.org/10.1128/jcm.00629-25
Primary Topic
Vector-borne infectious diseases
Type
article
Field-Weighted Citation Impact
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article

The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease

Elizabeth J. Horn, Peter Gwynne, A. Weber, Anna F. Hickman
Journal of Clinical Microbiology
Vector-borne infectious diseases
article

The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease

Elizabeth J. Horn, Peter Gwynne, A. Weber, Anna F. Hickman
article en

Abstract

During initial Lyme disease infection, existing diagnostic tests have poor sensitivity, resulting in a high number of false-negative tests. This is due to the lag between infection and a robust immune response capable of being detected by such tests. With a multiplexed array of nine unique antibody targets specific for <i>Borrelia burgdorferi</i>, interpreted by a proprietary machine learning algorithm, the InBios Lyme Detect Multiplex ELISA has the potential to increase diagnostic sensitivity within the first few weeks of infection, reducing the number of false-negative tests. Improving diagnostic sensitivity during early infection would reduce the risk of developing severe symptoms, including post-treatment Lyme disease.

Journal of Clinical MicrobiologyVol. 63(11)
Tufts University (US), Lyme Disease Association (US)
Openalex Percentile: Top 71%
Vector-borne infectious diseases
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The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease — Elizabeth J. Horn, Peter Gwynne, et al. · Journal of Clinical Microbiology (2025) | TGRS Research Map | TGRS