The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease
During initial Lyme disease infection, existing diagnostic tests have poor sensitivity, resulting in a high number of false-negative tests. This is due to the lag between infection and a robust immune response capable of being detected by such tests. With a multiplexed array of nine unique antibody targets specific for <i>Borrelia burgdorferi</i>, interpreted by a proprietary machine learning algorithm, the InBios Lyme Detect Multiplex ELISA has the potential to increase diagnostic sensitivity within the first few weeks of infection, reducing the number of false-negative tests. Improving diagnostic sensitivity during early infection would reduce the risk of developing severe symptoms, including post-treatment Lyme disease.
Authors
- Elizabeth J. Horn (ORCID: https://orcid.org/0000-0001-5928-9652)
- Peter Gwynne (ORCID: https://orcid.org/0000-0002-4111-5563)
- A. Weber
- Anna F. Hickman
Institutions
- Tufts University (US)
- Lyme Disease Association (US)
Publication Details
- Journal
- Journal of Clinical Microbiology
- Published
- 2025-10-09
- DOI
- https://doi.org/10.1128/jcm.00629-25
- Primary Topic
- Vector-borne infectious diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00