Isolation, identification and antibacterial activity of endophytes from the seeds of Panax japonicus

In this study, we investigated the method of P. japonicus seed disinfection, and the results showed that 75% alcohol disinfection for 30s and 1% HgCl2 disinfection for 9 min resulted in the lowest contamination rate of 3.33%, in which the greatest influence on the seed disinfection effect was the concentration of HgCl2. Meanwhile, 10 endophyte species of P. japonicus seeds were isolated and identified, Fusarium spp. and Alternaria alternata. were not the dominant species in the growth and development process of P. japonicus while Enterobacteriaceae and Pseudomonas sp. were the growth-promoting endophyte in the promotion of plant growth and development, and provide a theoretical reference for further research on the biological functions and active substances of endophyte in the seeds of P. japonicus.