Investigation of Phenolic‐Flavonoid Contents, Antioxidant Capacities, and Enzyme Inhibitory Properties of <i>Papaver Triniifolium</i> Extracts

Abstract Papaver triniifolium , a member of the Papaveraceae family Türkiye, was investigated for its phytochemical composition, antioxidant potential, and enzyme inhibitory properties. The underground part of the plant were extracted using various solvents (methanol, ethanol, methanol‐dichloromethane, ethyl acetate, dichloromethane, and n ‐hexane) through the maceration technique. The extracts were evaluated for their in vitro inhibitory effects on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α‐glycosidase enzymes. Additionally, antioxidant capacities were assessed using multiple assays, including DPPH, ABTS, FRAP, CUPRAC, and Fe 3 ⁺–Fe 2 ⁺ reduction methods. LC‐MS/MS analysis of the methanol extract revealed vanillic acid (927.60 µg/g) and vanillin (249.05 µg/g) as the major phenolic constituents. The methanol extract exhibited the strongest inhibitory activity against AChE (IC 50 : 62.10 ± 0.11 µg/mL) and α‐glucosidase (IC 50 : 44.77 ± 0.12 µg/mL). These results suggest that P. triniifolium may serve as a promising natural source of bioactive compounds with potential applications in managing neurodegenerative disorders, such as Alzheimer's and Parkinson's diseases, as well as metabolic conditions like Type 2 Diabetes Mellitus. The findings provide a pharmacological basis for further in vivo and clinical investigations.