Molecular chaperone effects on recombinant yield and binding characteristics of an ABA-specific scFv in Escherichia coli
This study highlights that molecular chaperone choice influences both structural fidelity and functional performance of ABA-scFv in E. coli. While pKJE7 favors high sensitivity, pTf16 yields highly specific and structurally stable antibodies. These findings establish a practical basis for developing low-cost ABA immunoassays with tailored performance for agricultural biotechnology.
Authors
- S.-L. Chen (ORCID: https://orcid.org/0000-0002-5904-4695)
- Bin Shan (ORCID: https://orcid.org/0000-0001-7800-0762)
- Qi Lu (ORCID: https://orcid.org/0000-0002-1725-5398)
- Guodong Mo (ORCID: https://orcid.org/0000-0003-4467-3058)
- Usman Rashid Malik (ORCID: https://orcid.org/0000-0002-8872-9749)
- Lin‐Li Lv (ORCID: https://orcid.org/0000-0003-4118-4304)
- Yang Luo (ORCID: https://orcid.org/0000-0001-9355-603X)
- Xinyu Yang (ORCID: https://orcid.org/0009-0004-4716-8641)
Institutions
- Guangxi Subtropical Crops Research Institute (CN)
- Ministry of Agriculture and Rural Affairs (CN)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-10-09
- DOI
- https://doi.org/10.3389/fbioe.2025.1643833
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Guangxi Province
- Guangxi Academy of Agricultural Sciences