INVESTIGATION OF THE EFFECT OF PARACETAMOL BIODEGRADATION PRODUCTS ON VEGETATIVE DEVELOPMENT AND DYNAMICS OF ACCUMULATION OF CARDIAC GLYCOSIDES IN THE LEAVES OF THE LILY OF THE VALLEY

The aim of the work is to study the effect of the biodegradation product of paracetamol on the vegetative development and dynamics of accumulation of cardiac glycosides in the leaves of the lily of the valley (Convallaria majalis L.), fam. Liliaceae using kinetic modeling. Materials and methods. The experiments were conducted in the Kirov region (2022) in accordance with the Guidelines for conducting registration tests of growth regulators (2016). The scheme of the experiment: the control area – natural watering with water; the test area – watering with a product of biodegradation of paracetamol obtained from paracetamol waste by a biotechnological method, the comparison area – watering with a growth stimulant "Zircon". The content of cardiac glycosides was determined by the spectrophotometric method based on the Balier reaction. The dynamics of changes in the size of seedlings, leaf mass and the content of cardiac glycosides in them were modeled by kinetic equations of the zero order with the determination of model parameters by the least squares method. Statistical processing of experimental data was carried out by the method of variance analysis using Excel 2019, Statistica 12.6, Statgraphics Plus 5.1 programs. Results and discussion. The biodegradation product of paracetamol increased the growth rate of lily of the valley seedlings by 47.0%, leaf weight by 92.8%, while the growth stimulant Zircon increased by 12.8% and 24.4%, respectively. The width of the leaf plate under the influence of the product under study increased by 1.11 times, the length – by 1.29 times compared with the growth stimulant "Zircon". The content of cardiac glycosides in the leaves increased by 11.43% and 3.81%, respectively, when treated with the product under study and the growth stimulant Zircon. Mathematical modeling of the dynamics of changes in the height of seedlings and accumulation of cardiac glycosides in leaves confirmed the linear nature of the processes. A nonlinear (parabola-wise) increase in the mass of medicinal plant raw materials with a zero order of equations has been established due to a linear increase in the rate of its accumulation over time. Conclusion. The biodegradation product of paracetamol significantly accelerates the growth of seedlings and leaves of the lily of the valley, an increase in leaf mass and the content of cardiac glycosides in them. The advantage of using this product in comparison with the analogue growth stimulant "Zircon" is shown. The data obtained can be used to form a dossier for registration of a paracetamol biodegradation product as a new plant growth stimulant.