Generation of equine induced pluripotent stem cells from cells of embryonic, perinatal and adult tissues
The developmental stage of the starting cell strongly influences their reprogramming potential in equine species. This has been suggested for human and other animal species, but direct comparison of equine cells from prenatal, perinatal and adult sources has not been reported before. Novel preliminary insight into the transcriptomic changes of different equine cell types during reprogramming, and on the effect of different culture conditions, can contribute improving the generation of eqiPSCs. While transgene-free methods are the goal, putative eqiPSCs are critical to enlarge our knowledge on animal iPSC biology.
Authors
- C. Rodellar
- Mary Murphy (ORCID: https://orcid.org/0000-0003-0971-7684)
- Frank Barry (ORCID: https://orcid.org/0000-0002-5535-002X)
- Alina Cequier (ORCID: https://orcid.org/0000-0002-1481-9805)
- Tarlan Eslami Arshaghi
- Ana Ivanovska (ORCID: https://orcid.org/0000-0001-5911-6882)
- Fiona Hollinshead (ORCID: https://orcid.org/0000-0002-3331-1126)
- Aisling O’Brien (ORCID: https://orcid.org/0000-0001-5520-9694)
- Laura Barrachina (ORCID: https://orcid.org/0000-0001-9818-508X)
Institutions
- Ollscoil na Gaillimhe – University of Galway (IE)
- Universidad de Zaragoza (ES)
- Centro de Investigación y Tecnología Agroalimentaria de Aragón (ES)
- Instituto de Investigación Sanitaria Aragón (ES)
- Colorado State University (US)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-10-08
- DOI
- https://doi.org/10.1186/s13287-025-04671-1
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission
- University of Galway
- Ministerio de Ciencia e Innovación
- Universidad de Zaragoza