The Determination of Salivary Mucin Concentration among 12 years old Children, Mongolia

Objective:The aim of this study was to assess the concentration of salivary mucin protein MUC5B and MUC7. Design:The study population consisted of 190 children aged 12 years old, Ulaanbaatar, Mongolia.We obtained according to the informed consent No.2022/3-03-36 of the Ethical Committee.The oral examination was done according to the recommendation of WHO.Saliva samples were collected between 9:00 AM and 11:00 AM among selected 81 children.Selected children abstained from eating and drinking for 2 h.Salivary mucin protein was analyzed by Sunlong Biotech Co., Ltd.Human Mucin-5B, Mucin-7, ELISA Kit (SL1210Hu, SL1211Hu) in the Medical Pathology Laboratory of MNUMS. Results:The prevalence and DMF/t score of dental caries were 88.6% and 2.79 among all children.The unstimulated salivary flow rate was 0.340.2ml/min among selected children, 0.410.22ml/min in control-I group, 0.360.19ml/min in control-II group and 0.260.17ml/min in the case group (p<0.05).The mean concentrations of MUC7 and MUC5B amounted to 0.380.22ng/ ml and 0.310.09ng/ml in the control I, 0.420.19ng/ml and 0.350.11ng/ml in the control II group, 0.450.07ng/ml and 0.380.13ng/ml in control group, respectively (p<0.001).Conclusions: Unstimulated salivary flow rate was significantly different between groups (p=0.018),but concentration of salivary MUC5B (p=0.96) and MUC7 (p=0.78)proteins was not significantly different.There was a significantly weak and negative relationship between dental caries and unstimulated salivary flow rate (p=0.018) and significantly weak and positive relationship between dental caries and concentration of salivary MUC5B and MUC7 proteins.