Time-dependent expression of neural oxidative and inflammatory biomarkers in ischemic stroke using a transient MCAO rat model
Ischemic stroke is globally acknowledged as a prominent reason for disability and mortalities. Oxidative stress, neuroinflammation, and autophagy have been implicated in its pathogenesis. A model of middle cerebral artery occlusion (MCAO) in male rats was conducted for this investigation and the brain cortex was sampled taken after at 1, 6, 12, 24, and 48 h of IRI. The results revealed time-dependent decreases in cortical GSH with rises in MDA and NO levels. Additionally, marked upregulations of inflammatory cytokines were observed and increased as time progressed. Besides, significant upregulations in TLR4, GFAP, Iba-1, and BDNF mRNA expressions were noticed along with increases in the P62 and decreases in LC3 immune expression levels. The pathological findings of cerebral sections showed vacuolations in the neuropil, perivascular edema, and shrinkage of neurons which also increased gradually by time. Taken together, these biomarkers could be reliable indicators for determination of time elapsed after IRI.
Authors
- Heba M. Elhessy (ORCID: https://orcid.org/0000-0002-0050-9953)
- Samah Fathy Ibrahim (ORCID: https://orcid.org/0000-0002-2824-3296)
- Ahmed Abdeen (ORCID: https://orcid.org/0000-0003-4907-955X)
- Rasha E. Azab
- Basma M. Hendam (ORCID: https://orcid.org/0000-0001-7420-6704)
- Hind A. Alzahrani (ORCID: https://orcid.org/0009-0008-9974-5665)
- Lamiaa M. Elabbasy (ORCID: https://orcid.org/0000-0003-1505-5357)
- Kasim Sakran Abass (ORCID: https://orcid.org/0000-0002-4202-783X)
- Hind Zafrah (ORCID: https://orcid.org/0009-0002-6987-6222)
- Majed Aldehri
- Ruba Almaghrabi
- Ola A. Habotta (ORCID: https://orcid.org/0000-0003-0067-2838)
- Mohamed Abomosallam (ORCID: https://orcid.org/0000-0002-8532-8210)
- Basma Othman
- Noor A Mohmand
Institutions
- University of Kirkuk (IQ)
- Princess Nourah bint Abdulrahman University (SA)
- Alfaisal University (SA)
- Mansoura University (EG)
- Benha University (EG)
- Herat University (AF)
- Almaarefa University
- Al Baha University (SA)
- University of Tabuk (SA)
- King Khalid University (SA)
Publication Details
- Journal
- Scientific Reports
- Published
- 2025-10-08
- DOI
- https://doi.org/10.1038/s41598-025-08178-w
- Primary Topic
- Neuroinflammation and Neurodegeneration Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- AlMaarefa University
- Science and Technology Development Fund
- Princess Nourah Bint Abdulrahman University
- King Khalid University
- Deanship of Scientific Research, Princess Nourah Bint Abdulrahman University