Extractive values, quantitative phytochemical analysis, and FTIR spectroscopic profiling of Careya arborea Roxb. root

This study aimed to explore the phytochemical profile, extractive values, quantitative assessment of bioactive compounds, anti-inflammatory activity and FTIR characterization of Careya arborea Roxb. root. The evaluation of extractive values revealed the highest yields when using polar solvents such as methanol ethanol and water underscoring their efficiency in extracting polar phytochemicals. Initial phytochemical screenings verified the presence of alkaloids, phenols, flavonoids, tannins, saponins, terpenoids, phytosterols, and cardiac glycosides, with variations observed based on the polarity of the solvents. Quantitative analyses further confirmed that significant levels of phenolic compounds, flavonoids tannins and terpenoids were present. In- vitro anti-inflammatory activity showed maximum amount of inhibition of protein denaturation increasing from 57.6% at 100 µg/mL to 92.3% at 500 µg/mL reaching near that of Prednisone, Aceclofenac. The FTIR analysis revealed characteristic absorption peaks associated with O-H C-H C=O C=C, and C-O bonds supporting the presence of phenols, flavonoids glycosides and terpenoids. A review of earlier studies has shown that while the bark and fibre of Careya arborea Roxb. have been investigated, these findings collectively provided a scientific basis for the traditional uses of C. arborea Root underscoring its potential as a source of pharmacologically effective substances.