Comparative Study of 3D Reconstruction Techniques on Rhinoceros Iguana Skull by Utilizing Computer Tomography Scans

Abstract The rhinoceros iguana is an endangered species native to the Caribbean, currently listed on the International Union for Conservation of Nature (IUCN) Red List. While most available studies focus on its ecology and conservation, there is a lack of detailed anatomical descriptions, especially of the skull, which limits clinical, educational, and research applications in veterinary medicine. Computed tomography (CT) and three-dimensional (3D) reconstructions, provides a non-invasive and precise method to identify and annotate anatomical structures in reptiles. This study aimed to evaluate and illustrate the anatomical fidelity and utility of different CT-based reconstruction techniques, using Cyclura cornuta cornuta as a representative model. We applied three post-processing methods-Volume Rendering (VR3D), Maximum Intensity Projection (MIP), and Multiplanar Reformatting with Volume Rendering (MPR-VR)-to two female adult specimens. Our results demonstrate that each technique offers unique advantages in visualizing cranial features, and that combining them improves anatomical interpretation. This work provides a practical imaging reference for reptile skull anatomy and supports future applications in clinical diagnostics, comparative morphology, and exotic animal education.