Dual Action Defense - An In Vivo Assessment of Salivary Fluoride Dynamics Post Fluoride Varnish-Povidone Iodine Application

Background: Fluoride varnish is widely used as a caries-preventive agent due to its ability to enhance enamel remineralization and inhibit demineralization. The adjunctive use of Povidone-Iodine (PVP-I), a broad-spectrum antimicrobial, may prolong fluoride retention in the oral cavity by reducing cariogenic bacterial activity. Aim: To estimate salivary fluoride concentration following topical application of fluoride varnish with Povidone-Iodine at different time intervals. Materials and Methods: Fifteen healthy children aged 7?10 years were enrolled. After baseline unstimulated saliva collection, PVP-I was applied for one minute and rinsed off, followed by application of fluoride varnish. Saliva samples were collected at baseline, 24 hour and 48 hours. Fluoride concentration was estimated using a fluoride ion-specific electrode. Data were analyzed using repeated measures ANOVA (p < 0.05). Results: Mean baseline fluoride level was 0.35 ppm. Following FV + PVP-I application, fluoride levels rose significantly at 24 hours (1.65 ppm), gradually decreased at 48 hours (1.32 ppm). The rise at 24 h and 48 h compared to baseline was statistically significant (p < 0.05). Conclusion: Application of fluoride varnish with Povidone-Iodine significantly elevated salivary fluoride concentration for up to 48 hours. This combination may provide dual benefits of antibacterial action and enhanced fluoride availability, supporting its use as a preventive strategy in Pediatric dentistry.