Development and Validation of an HPLC-PDA Method for NMN Quantification in Commercial Pet Foods
Given NMN’s (Nicotinamide mononucleotide, NMN) potential pet health benefits and wide use in pet foods, the lack of standardized detection methods hinders quality control. This study developed and validated a simple, rapid HPLC (High-Performance Liquid Chromatography, HPLC) method for NMN determination in pet foods. The method showed good linearity (5–500 μg/mL), LOD (1.0 mg/kg), LOQ (2.0 mg/kg), precision, stability, reproducibility, and spiked recoveries (97.3–109%, RSD < 6.0%). And most tested commercial samples met the standards. This method is simple, efficient, and accurate, supporting pet food NMN detection, quality control, regulation, and pet health protection.
Authors
- Y. Gloria Meng (ORCID: https://orcid.org/0009-0008-8762-0785)
- Tao Lan (ORCID: https://orcid.org/0000-0001-5306-5826)
- Cunhao Li
- Jingxuan Zhang (ORCID: https://orcid.org/0000-0002-1405-3977)
- Lihong Wang (ORCID: https://orcid.org/0000-0001-9915-8868)
Institutions
- Jiamusi University (CN)
- China National Institute of Standardization (CN)
- Detection Limit (United States) (US)
- Hebei Science and Technology Department (CN)
Publication Details
- Journal
- Applied Sciences
- Published
- 2025-10-08
- DOI
- https://doi.org/10.3390/app151910797
- Citations
- 1
- Primary Topic
- Coffee research and impacts
- Type
- article
- Field-Weighted Citation Impact
- 1.39
Funders
- National Key Research and Development Program of China