miR-302d-3p expression and genotype are associated with CMV replication after allogeneic haematopoietic stem cell transplantation

Cytomegalovirus (CMV) reactivation is a common complication after allogeneic haematopoietic stem cell transplantation (HSCT). It occurs in over a third of alloHSCT recipients, and is a major source of post-transplant mortality. miRNAs are small non-coding RNA molecules that are important regulators of gene expression. They are often dysregulated during disease, including transplant-related complications. Due to their presence in all body fluids, they can be used as potential biomarkers. In this study, we performed miRNA profiling to find differentially expressed host miRNAs in serum that could be linked to CMV reactivation after HSCT. CMV reactivation was confirmed by detection of CMV DNA in serum. We identified two miRNAs, miR-302d-3p (p < 0.001) and miR-6721-5p (p = 0.018), as differentially expressed between day + 30 and + 90 after HSCT in patients with CMV reactivation. Of these, miR-302d-3p was confirmed as overexpressed on day + 30 in a verification analysis performed on an independent cohort of patients (p = 0.027). Furthermore, we analysed two genetic variants in the gene coding for miR-302d-3p. We found one of them, rs13136737, to be significantly associated with CMV reactivation (p = 0.015). In conclusion, our study showed that miR-302d-3p is dysregulated and that its genetic variant rs13136737 may be important in development of CMV reactivation after HSCT.

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Publication Details

Journal
Human Immunology
Published
2025-10-08
DOI
https://doi.org/10.1016/j.humimm.2025.111599
Primary Topic
Cytomegalovirus and herpesvirus research
Type
article
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article

miR-302d-3p expression and genotype are associated with CMV replication after allogeneic haematopoietic stem cell transplantation

Barbara Nasiłowska‐Adamska, Wojciech Fidyk, Anna Czyż, Małgorzata Sobczyk‐Kruszelnicka et al.
Human Immunology
Cytomegalovirus and herpesvirus research
article

miR-302d-3p expression and genotype are associated with CMV replication after allogeneic haematopoietic stem cell transplantation

Barbara Nasiłowska‐Adamska, Wojciech Fidyk, Anna Czyż, Małgorzata Sobczyk‐Kruszelnicka, Jagoda Siemaszko, Tomasz Wróbel, Rachel E. Crossland, Sebastian Giebel, Piotr Łacina, Grzegorz Władysław Basak, Maria Bieniaszewska, Patrycja Skowrońska, Iwona Solarska, Agnieszka Szeremet, Maciej Majcherek, Katarzyna Bogunia‐Kubik, Agnieszka Tomaszewska
article en

Abstract

Cytomegalovirus (CMV) reactivation is a common complication after allogeneic haematopoietic stem cell transplantation (HSCT). It occurs in over a third of alloHSCT recipients, and is a major source of post-transplant mortality. miRNAs are small non-coding RNA molecules that are important regulators of gene expression. They are often dysregulated during disease, including transplant-related complications. Due to their presence in all body fluids, they can be used as potential biomarkers. In this study, we performed miRNA profiling to find differentially expressed host miRNAs in serum that could be linked to CMV reactivation after HSCT. CMV reactivation was confirmed by detection of CMV DNA in serum. We identified two miRNAs, miR-302d-3p (p < 0.001) and miR-6721-5p (p = 0.018), as differentially expressed between day + 30 and + 90 after HSCT in patients with CMV reactivation. Of these, miR-302d-3p was confirmed as overexpressed on day + 30 in a verification analysis performed on an independent cohort of patients (p = 0.027). Furthermore, we analysed two genetic variants in the gene coding for miR-302d-3p. We found one of them, rs13136737, to be significantly associated with CMV reactivation (p = 0.015). In conclusion, our study showed that miR-302d-3p is dysregulated and that its genetic variant rs13136737 may be important in development of CMV reactivation after HSCT.

Human ImmunologyVol. 86(6)
Medical University of Warsaw (PL), Wroclaw Medical University (PL), Instytut Hematologii i Transfuzjologi (PL), The Maria Sklodowska-Curie National Research Institute of Oncology (PL), Ludwik Hirszfeld Institute of Immunology and Experimental Therapy (PL), Gdańsk Medical University (PL), Newcastle University (GB), Polish Academy of Sciences (PL)
Openalex Percentile: Top 62%
Cytomegalovirus and herpesvirus research
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