Identification of Rose Viral Species Using RT-PCR Technology

As an important ornamental plant, rose is severely affected by virus infections, which significantly impair its growth and ornamental value. Accurate identification of virus species is crucial for the prevention and control of viral diseases. To clarify the main virus species infecting roses in Jingmen, this study employed RT-PCR technology to identify rose viruses, providing a theoretical basis for the investigation and comprehensive prevention/control of rose viruses in Jingmen. Rose petal and leaf samples suspected of viral infection were collected from four regions in Jingmen. Rose RNA was extracted using methods such as Trizol and RNA extraction kits. Specific primers were designed for common viruses including Tobacco Mosaic Virus (TMV) and Rose Mosaic Virus 2 (RIV2), followed by RT-PCR amplification. The sample bands were detected using agarose gel electrophoresis and a gel imaging analysis system to determine the virus species. Among the 40 samples, Apple Stem Grooving Virus (ASGV), Prunus Necrotic Ringspot Virus (PNRSV), Rose Cryptic Virus 1 (RoCV1), and Rose Mosaic Virus 2 (RIV2) were detected. The results showed that RT-PCR technology can efficiently identify rose virus species; RIV2 and ASGV were found to be the main viruses infecting roses in Jingmen. This study enriches the research and investigation data for the future prevention/control of rose viral diseases and the study of their pathogens.