FEASIBILITY OF APPLICATION OF A POTENTIOMETRIC BIOSENSOR BASED ON CREATININE DEIMINASE FOR NON-INVASIVE CREATININE ANALYSIS

The paper investigates the possibility of application of potentiometric biosensor based on creatinine deiminase for non-invasive analysis of creatinine in urine, sweat and saliva. The effect on the signal of various substances that can potentially affect the biosensor response and are contained in the urine of healthy people or patients with kidney diseases has been studied. It was shown that the contribution of these components to the total signal is minimal and will not significantly affect the accuracy of biosensor measurements. The stability during storage of the biosensor was more than 120 days. The principle possibility of creatinine determination by using a biosensor based on creatinine deiminase in saliva and sweat was shown, while the lowest creatinine concentration that can be determined was 50 μM. The developed biosensor demonstrates certain advantages in determining creatinine concentration in urine samples compared to the traditional colorimetric Jaffe method, which requires expensive equipment and careful control of reaction parameters to reduce the influence of interfering substances, as well as a complex measurement procedure.