Decolourization of Malachite Green Dye by Bacteria Isolated from Drainage Effluent

The widespread use of synthetic dyes, particularly malachite green (MG), in various industries has led to significant environmental pollution, necessitating effective and eco-friendly remediation strategies.This study focused on the isolation and identification of bacterial strains capable of degrading malachite green dye.A total of seven bacterial isolates were obtained from a drainage canal of New Market, Bhopal (Madhya Pradesh).Preliminary screening on dye-containing media identified two potent isolates Pseudomonas otitidis and Aeromonas hydrophila, exhibiting significant degradation capabilities.Biodecolorization Assay confirmed that isolate Pseudomonas otitidis achieved the highest decolorization efficiency of 80% in optimized condition (72 hours, 35C), while Aeromonas hydrophila reached 76% under similar conditions for the malachite green dye.BLASTn analysis confirmed 99.86 and 100 percentage sequence similarity with known bacterial strains.Overall, the findings highlight Pseudomonas otitidis and Aeromonas hydrophila as promising bacteria for the bioremediation of malachite green contaminated environments, with Pseudomonas otitidis showing exceptional efficiency and potential for application in sustainable wastewater management strategies.