PacBio single-molecule long-read transcriptome sequencing and analysis of somatic embryogenesis in Picea mongolica

This study establishes the first reference-quality transcriptome database for <i>P. mongolica</i> using PacBio long-read sequencing, providing essential genomic resources for this non-model species. Our findings not only enhance the understanding of molecular mechanisms in somatic embryogenesis but also lay a foundation for future functional genomics research, including gene validation and molecular marker-assisted breeding. These results have significant theoretical and practical implications for the conservation and sustainable utilization of this endangered conifer.