Probiotic Properties of Lactic Acid Bacteria Isolated from Breast Milk

The present study aimed to characterize different isolates from human breast milk and their in vitro probiotic potentialities. 16S rDNA from each isolate was sequenced. The isolates were characterized by determining their resistance to gastrointestinal conditions (pH, bile salts, and lysozyme), antibiotic resistance, antimicrobial activity, biofilm formation, exopolysaccharide production, and carbohydrate fermentation profile. Five isolates were obtained with probiotic properties belonging to the species of Limosilactobacillus fermentum and Lactiplantibacillus plantarum isolated from human breast milk. Isolates LT1512 and LM1563C presented the best probiotic properties due to high percentages of survival under the gastrointestinal conditions, antimicrobial properties against Escherichia coli, resistance to twelve antibiotics, and biofilm formation. Conclusions. This study confirmed that the breast milk of women with a healthy food pattern is a good source of isolation from lactic acid bacteria. Milk carries a microbiota with potential probiotic properties, making it the source of probiotics and the focus for further research.