Cobalt Chloride and Nano Zinc as Effectors of <i>Brassica Oleracea</i> Var. Gongylodes L Propagation and Production of Active Compounds <i>in Vitro</i> Culture

Abstract Plants under stress synthesize different active compounds and these ingredients gradients released by plants can be influenced under unconventional conditions, and are usually considered secondary metabolism compounds. Brassica oleracea var. gongylodes is considered a valuable plant, as it is the source of the most valuable natural active compounds. Furthermore, it is appropriate for in vitro intact plant propagation techniques. The goal of this study is to elucidate through a comprehensive analysis of B. oleracea propagation by applying different concentrations of plant hormones and propagate and induce callus formation in vitro culture and the relationship of elicitation effect by cobalt chloride and nano zinc and producing secondary metabolites compounds. The explant of B. oleracea was grown on media containing a mix of hormones 1, 2, 3, of BA and (0.5) NAA mg per liter. BA (2.0) with, NAA (0.5) mg had a considerable effect on number of explants 13.0 shoots.explant −1 . Shot tip explant, BA 2. mg. and maximum effect on the shoots’ length, that reached 7 cm and 13.0 number of leaves. Nano zinc 1mg/L applied increased the shoots in addition to the number of leaves explants and the accumulation of antioxidant glutathione of B. oleracea to 28.227 μg/ml after elicitation with cobalt chloride and to 24.777 μg/ml after applying Nano Zinc. The application of different types of elicitors like chemical solutions such as Cobalt chloride and nano zinc have often been reported to affect plant accumulation partially in the active compounds. These effects can enhance plant secondary metabolism growth rate in many plant processes to adapt to the environment.