Dosage of phenolic compounds and evaluation of anti-inflammatory activity in vivo of Rubia tinctorum extract

This study is part of the search for new biologically active molecules of plant origin. The aim of this study is the in vivo evaluation of the anti-inflammatory activity of the hydro-methanolic extract of the underground part (roots) of Rubia tinctorum from the Wilaya of Chlef (Algeria). The quantitative estimation of polyphenols, flavonoids, and tannins by the colorimetric method showed that the extract studied is rich in these compounds, with contents equal to 199.17 ± 0.074 mg GAE/g, 53.65 ± 0.042 mg QE/g, and 45.74 ± 0.033 mg CE/g, respectively. The anti-inflammatory activity was evaluated using the model of paw edema induced by the intraperitoneal (IP) route in mice (Morini strain) by carrageenan. The results obtained were compared to those of the reference treatment (diclofenac sodium). The evaluation of the percentage of inhibition of the hydro-methanolic extract of the root of Rubia tinctorum at doses of 25, 50, and 100 mg/kg significantly prevented paw edema (p