Blackthorn juice from central Italy exhibits selective anticancer activity by inducing apoptosis and autophagy in gastric carcinoma cells

Blackthorn (<i>Prunus spinosa</i> L.) juice, a polyphenol-rich blend derived from the Marche region of Italy, was chemically characterized and evaluated for its selective effects against gastric cancer cells. The juice contained a mix of polyphenols, including neochlorogenic acid, cyanidin-3-glucoside, rutin, and smaller molecules - such as gallic, caffeic, and vanillic acids - characterized by high absorption rates. <i>In vitro</i>, the juice significantly reduced the viability of AGS and KATO III gastric cancer cells, while sparing non-tumorigenic GES-1 cells. In malignant cells, the juice activated Caspase-3, upregulated Bax, and downregulated Bcl-xL, while also modulating autophagy by increasing LC3B-II levels and decreasing Beclin-1. Mitochondrial impairment was confirmed by cardiolipin loss and cytochrome c release into the cytoplasm. These findings support the potential of <i>Prunus spinosa</i> L. juice as a selective, innovative functional beverage for gastric cancer prevention, acting beyond nutrition but before the need for medical intervention.