MOLECULAR IDENTIFICATION OF CACTACEAE PLANT USING rbcL DNA BARCODE

DNA barcoding is a technique of employed in short DNA sequences of the standard genome segment to identify species on a large scale. Compared to morphology-based taxonomy identification, which is challenging and prone to subjective biases, DNA barcoding is becoming more and more popular due to its ease of use and high accuracy rate. Thestandard chloroplast DNA barcode a terrestrial plant, which was developed by the Consortium for the Barcode of Life (CBOL)plant working group, has to be evaluated for knowing variation of plant species. The amplicons were created by amplifying the isolated genomic DNA using the rbcL gene in a PCR. The recovered amplicons, which are ranged in length from 500 to 800 bp, were subjected to sequencing for the rbcL gene area. Therefore, in the present investigation the rbcL marker's potential for identifying plants in the cacti family is undertaken. To assess the rbcL gene's discriminating capacity, maximum neighbourhood tree analysis was used. The result obtained from DNA barcoding matches up to the genus level including Turbinicarpus and species up to 99 % but these species though it is having 98% rbcL gene similarly with existing species it has 1 % of individual specific genomic component that supports to be identified a new variant species hence these Cactaceae plant family species is considered as new species.