A Review on Various Analytical Method Development and Validation for Simultaneous Estimation of Levo-Salbutamol and Ipratropium Bromide in Bulk and Pharmaceutical Dosage Form by RP-HPLC

For the simultaneous measurement of levosalbutamol and ipratropium bromide, a quick and sensitive reverse phase high performance liquid chromatography (RP-HPLC) approach has been devised. Chromatographic separation was accomplished using a mobile phase made up of 0.01M potassium dihydrogen phosphate and ethanol (pH was corrected to 3.0 using O-phosphoric acid) on a reverse phase Enable C18 column (250 X 4.6 mm, 5 µm). A UV detector set to 245 nm was used for detection after the mobile phase was pumped at a flow rate of 1.0 ml/min. The suggested approach could be used for routine quality control analysis for the simultaneous determination of levosalbutamol and ipratropium bromide in pharmaceutical dosage forms since it was found to be easy to use, quick, accurate, precise, and repeatable.