The endoplasmic reticulum-associated degradation machinery selectively degrades stress-induced TIN1 during stress recovery
The unfolded protein response (UPR) signaling pathway is activated by the accumulation of misfolded proteins in the endoplasmic reticulum (ER) and stimulates the production of ER chaperones to restore ER proteostasis. However, how UPR-induced proteins return to their pre-stress levels upon removal of ER stress remains unknown. TUNICAMYCIN-INDUCED 1 (TIN1) is an Arabidopsis (Arabidopsis thaliana) protein that is normally expressed in pollen but is rapidly induced by ER stress in vegetative tissues. Here, we show that the ER-stress-induced TIN1 is rapidly degraded in the UPR recovery phase. We found that TIN1 degradation depends on its asparagine-linked glycans and requires both EMS-mutagenized bri1 suppressor 5 (EBS5) and EBS6 for its recruitment to the ER-associated degradation (ERAD) complex. Loss-of-function mutations in the core component of this Arabidopsis ERAD complex greatly stabilize TIN1. Interestingly, 2 other UPR-induced proteins that are coexpressed with TIN1 remained stable upon ER-stress removal, suggesting that rapid degradation during the stress-recovery phase likely applies to a subset of UPR-induced proteins. Further investigation is needed to uncover the mechanisms by which the ERAD machinery selectively degrades UPR-induced ER proteins.
Authors
- Yongwu Chen (ORCID: https://orcid.org/0000-0002-8692-7717)
- Linchuan Liu (ORCID: https://orcid.org/0000-0002-8669-7202)
- Liangguang Leo Lin (ORCID: https://orcid.org/0000-0003-1414-610X)
- Zhihui Ma (ORCID: https://orcid.org/0000-0001-8715-0754)
- Jiarui Wu (ORCID: https://orcid.org/0000-0002-1617-6110)
- Congcong Zhang (ORCID: https://orcid.org/0009-0002-1551-551X)
- Jianjun Zhang (ORCID: https://orcid.org/0000-0003-3798-4114)
- Yi Wang (ORCID: https://orcid.org/0000-0003-0797-5209)
- Jianming Li (ORCID: https://orcid.org/0000-0003-3231-0778)
- Pengcheng Wang (ORCID: https://orcid.org/0000-0001-6043-4132)
- Juan Mao (ORCID: https://orcid.org/0000-0003-0524-9895)
Institutions
- South China Agricultural University (CN)
- Hong Kong Baptist University (HK)
- Chinese University of Hong Kong (HK)
- University of Michigan (US)
- Southern University of Science and Technology (CN)
- Hong Kong Jockey Club (HK)
- Center for Excellence in Molecular Plant Sciences (CN)
- Guangdong Academy of Forestry (CN)
- University of Chinese Academy of Sciences (CN)
- State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources (CN)
Publication Details
- Journal
- PLANT PHYSIOLOGY
- Published
- 2025-09-30
- DOI
- https://doi.org/10.1093/plphys/kiaf489
- Citations
- 1
- Primary Topic
- Endoplasmic Reticulum Stress and Disease
- Type
- article
- Field-Weighted Citation Impact
- 1.15
Funders
- Hong Kong Baptist University
- National Natural Science Foundation of China
- Zhejiang University
- Arizona Biomedical Research Commission