Simultaneous Determination of Pharmaceuticals Using Two UV-Based Methods with Different Diluents: Comparative Analysis via ANOVA and Greenness Assessment

In this work, a UV spectrophotometric approach that is accurate, precise, and environmentally friendly Is proposed for concurrent quantification of Ritonavir and Nirmatrelvir in combination pharmaceutical form. While treating COVID-19, these antiviral medications are essential. The Q-absorbance method was created and validated in accordance with ICH criteria, and the simultaneous estimation method is constructed utilizing two distinct solvents. After being evaluated for linearity, accuracy, precision, robustness, and sensitivity, the suggested techniques showed great reproducibility and high correlation coefficients (r2 = 0.999). One-way ANOVA statistical comparison verified that the devised methodologies were reliable and consistent for pharmaceutical analysis, with no significant differences found.Furthermore, the Green Analytical Procedure Index (GAPI), AGREE, and BAGI criteria were used to assess the Ecological sustainability of these methods. The results demonstrated that the suggested approaches provide a greener substitute for traditional analytical procedures by drastically lowering solvent usage and the production of hazardous waste. The proposed UV spectrophotometric approaches offer a straightforward, economical, and dependable solution for the routine quality evaluation of Ritonavir and Nirmatrelvir in dosage forms.