Phytochemical screening, antioxidant power and quantitative analysis by HPLC of isolated flavonoids from Rosemary

The aim of this study was to evaluate of phytochemical, total phenolic content, total flavonoid content, total alkaloid content, total saponins content, total tannin content, the antioxidant activity and quantitative analysis of the isolated flavonoids by HPLC of Rosemary. The plant is known to contain phenolic chemicals, flavonoids, tannins, glycosides, alkaloids, terpenes, sterols, and saponids, according to preliminary phytochemical results. After quantitative estimation of secondary metabolites, the greatest concentration of total phenolic content 166.8 mg/100g, flavonoids 84.9 mg/100g, total alkaloids content 4.58 %, total tannins content 3.66 % and total saponins content 1.25 %. Results of HPLC showed p-coumaric acid 80.25 ppm, apigenin 30.59 ppm, kaempferol 44.8 ppm, ferulic acid 12.98 ppm, quercetin 51.58 ppm, sinapic acid 12.6 ppm, and rosmaric acid 30.9 ppm. Results showed the Rosemary have a reducing power capable of reducing Scavenge free radicals compared to ascorbic acid.