Endoboost plus is the medium of choice for the cell mass expansion of ecfc isolated from adult blood

Endothelial colony-forming cells (ECFC) have a powerful clonogenic and reparative potential, which makes them a promising material for cell therapy, experimental biology and medicine. The ability to rapidly expand the cell mass is the key to the use of ECFCs in these areas. We are developing the composition of nutrient medium for EndoBoost and EndoBoost Plus endothelial cells. Endothelial cell growth medium2 (EGM2) is recognised as the ‘gold standard’ in ECFC cultivation. The aim of our study was to comparatively evaluate the efficacy of EGM2, EndoBoost and EndoBoost Plus nutrient media for ECFC culture growth. Maximum proliferative activity of ECFC was detected in EndoBoost Plus medium, EndoBoost was found to be less active and the lowest result corresponded to EGM2. Thus, EndoBoost Plus is the preferred medium for cell culture of ECFCs isolated from adult peripheral blood.