Physically adsorbed coatings based on chitosan for electrophoretic separation of biologically active substances

Coatings of the inner walls of a quartz capillary based on cationic high-molecular chitosan with a deacetylation degree of 95% were formed. The dependence of the electroosmotic flow rate on the pH of the background electrolyte was studied, and the stability of the coating under the influence of various solvents was assessed. The results were compared with another cationic coating based on poly(diallyldimethylammonium chloride) (PDADMAC). It was shown that when separating amino acids, catecholamines, and organic acids, the formed coatings based on chitosan are slightly inferior in efficiency to coatings made of PDADMAC, but provide a higher resolution of the studied biologically active analytes. It was found that chitosan on the inner walls of a quartz capillary promotes an increase in enantioselectivity in the separation of β-blocker enantiomers (carvedilol, propranolol, sotalol) in the presence of (2-hydroxypropyl)-β-cyclodextrin in the background electrolyte, as well as non-steroidal anti-inflammatory drugs (ketoprofen and ketorolac) using vancomycin as the second chiral selector.