Molecular Characterization of Recombinant Inbred Lines (RILs) in Cauliflower (Brassica oleracea var. botrytis L.)

Cauliflower is an important vegetable crop grown commercially in India. Information on molecular markers along with linkage map construction is a prerequisite for molecular breeding. The present investigation was carried at the Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India, to study the variability of 66 recombinant inbred lines (RILs) developed in downy mildew resistance programme using 119 SSR markers. Among these, SSR markers, six were found to be polymorphic between the parents and finally five markers were polymorphic among the RILs. The cluster analysis using the UPGMA method showed six clusters at a 0.38 Jaccard dissimilarity coefficient. The clustering of RILs allocated 12 RILs outside cluster of both parents revealing differentiation of the RILs studied. The cluster I had maximum number of RILs (37) while cluster VI had only one RIL. It is concluded that these five markers i.e., Na12G12, Ol11G11, FIT0043, BoSF 376, and BoSF 2079 can further be used to link different trait in cauliflower.